Biotechnology and Biomedical Engineering
AMRITA VISHWA VIDYAPEETHAM
Introduction
The branch of biomedicine concerned with the structure and function of the immune system, innate and acquired immunity, the bodily distinction of self from no self, and laboratory techniques involving the interaction of antigens with specific antibodies.
1. To introduce the principle of precipitation reactions and differentiate it from agglutination reactions
2. To demonstrate the purification protocols of antibodies for use in research and diagnostics
3. To understand the concepts of mouse Euthanasia. To learn the basic procedures involved in rodent dissection and how to identify and remove lymphoid organs.
4. To understand the basic principles in fragmentation of IgG to a monovalent Fab fragment using Papain digestion. The cleavage of antibodies using the Proteolytic enzyme is first studied by porter in 1959. IgG is cleaved by Papain to give Fab fragments
5. To understand the principles of IgG fragmentation to a divalent F(ab')2 fragment.
2. To demonstrate the purification protocols of antibodies for use in research and diagnostics
3. To understand the concepts of mouse Euthanasia. To learn the basic procedures involved in rodent dissection and how to identify and remove lymphoid organs.
4. To understand the basic principles in fragmentation of IgG to a monovalent Fab fragment using Papain digestion. The cleavage of antibodies using the Proteolytic enzyme is first studied by porter in 1959. IgG is cleaved by Papain to give Fab fragments
5. To understand the principles of IgG fragmentation to a divalent F(ab')2 fragment.
- UG
- B.Tech./ B.E in Biotechnology
- PG
- PG in Biotechnology/Microbiology/Bioinformatics/Biomedical Science
The syllabi of this lab aligns to the following disciplines.
- UG in Biotechnology/Microbiology/Bioinformatics/Biomedical Science
- PG in Biotechnology/Microbiology/Bioinformatics/Biomedical Science
No university information available.
No reference books available.