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Experiments for Review

Experiments in Biotechnology and Biomedical En

Bioinformatics and Computational Biology Virtual Lab

Amrita Vishwa Vidyapeetham, Coimbatore

1. Basic machine learning using the WEKA tool
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10.To analyze the sequence of a given DNA and find out sequence composition
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2. Browsing genetic variation databases such as dbSNP, CLIN Var.
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3. Similarity search using BLAST.
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4. Visualization of Protein Structure
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5. Molecular Dynamics Simulation: GROMACS
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6. Basic Algorithms in Bioinformatics
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7. Conversion of sequence from one database format to another using file format converter
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8. An introduction to Next Generation Sequencing (NGS)
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9. To find out the number of times a sequence is repeated in a given DNA sequence
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Biosensor Virtual Lab

Amrita Vishwa Vidyapeetham, Coimbatore

1. Design and construction of a biosensor.
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10. Microfluidic MFC
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2. Demonstration of exoelectrogenesis using Caulobacter crescentus
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3. Deriving the I-V curve
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4. Impedance/resistance experiment using source meter
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5. Efficiency of sacrificial (aluminum, copper, manganese) and non-sacrificial electrodes (carbon, graphite) in MFC
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6. Proton exchange membranes (PEM)
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7. Phototrophic Microbial Fuel Cell using Chroococcus sp.
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8. Modular assembly of MFC to increase voltage or current.
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9. MFC in wastewater treatment
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Biochemistry Virtual Lab

Dayalbagh Educational Institute, Agra

1. Analyze quality and quantity of fats
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2. Analyze quality and quantity of DNA, RNA
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3. Quantitative estimation of proteins
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4. Preparation of phosphate buffer and Carbonate-Bicarbonate buffer
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Techniques in Immunology and Microbiology Lab

IIT Guwahati

1. Isolation and Microscopic Visualization of T cells and B cells
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10. Cloning and expression of GFP sequence/gene in the expression vector
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2. Flow cytometry of the immune cells
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3. Western blot analysis of the cell lysate using antibody
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4. 2D gel electrophoresis of the egg proteins
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5. Immunoprecipitation of protein from cell lysate using antibody
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6. Enzyme-linked immunosorbent assay (ELISA) using protein and antibody
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7. Study of different stages of Chick Embryo
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8. Effect of physical and chemical environment on growth
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9. Determination of minimal inhibitory concentration (MIC) of an antibiotic
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Experimental Biochemistry Virtual Lab

IIT Kharagpur

1. Computation of various physical and chemical properties of Proteins and DNA from their primary sequence.
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10. Kinetic characterization of protein-ligand binding by surface plasmon resonance (SPR).
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2. DNA amplification by polymerase chain reaction (PCR).
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3. DNA analysis by agarose gel electrophoresis.
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4. pH-titration of amino acids and small peptides, and estimation of isoelectric point (pI).
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5. Protein analysis by polyacrylamide gel electrophoresis (PAGE).
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6. Protein purification by affinity chromatography.
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7. Protein purification by size exclusion chromatography (SEC).
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8. Protein-DNA interaction analysis by electrophoretic mobility shift assay (EMSA).
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9. Thermodynamic characterization of protein-ligand binding by isothermal titration calorimetry (ITC).
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